Regeneration of Pinus halepensis (Mill.) through Organogenesis from Apical Shoot Buds

Organogenesis and somatic embryogenesis have been widely applied as the two main regeneration pathways in plant tissue cultures. However, recalcitrance is still restriction clonal propagation of many woody species, especially conifers. They undergo a “phase change” that leads to significant loss vegetative capacity, reducing aptitude tissues organs be regenerated vitro beyond this point. In line with this, mature conifer trees has long-cherished goal laboratories worldwide. Based on previous works Pinus species from adult trees, we now present data about culture apical shoot buds an attempt induce organogenesis clone Aleppo pine (Pinus halepensis). Reinvigorated axillary shoots were submitted conditions usually through manipulation media, including use auxins such 2,4-Dichlorophenoxyacetic acid 1-Naphthaleneacetic acid, cytokinins (6-benzyladenine kinetin), phytosulfokine (50, 100, 200 nM). Although embryos could not obtained, embryogenic-like was produced, followed by emergence actively proliferating non-embryogenic calli. Variations consistence, texture, color calli observed; those arising media containing phytosulfokine. shoots, induced 22 or 44 µM 6-benzyladenine, obtained acclimatized, phenotypically normal plants obtained.